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The maternal androgens testosterone and androstenedione are in the eggs of American kestrels in increasing concentration as the eggs are laid. This may be a method of influencing offspring phenotype by affecting immunity, survival, hatchability, and secondary sexual characteristics. My thesis project consists of injecting American kestrel clutches with one of three different designated doses of testosterone and androstenedione. The effects of these anabolic steroids on offspring phenotypes will be recorded and analyzed. Also, a new efficient and affordable method of analyzing bone alkaline phosphatase will be tested.